Sigma-Aldrich Introduces the TargeTron(TM) Gene Knockout System

Press Release:

Sigma-Aldrich Introduces the TargeTron(TM) Gene Knockout System

Press Release
News Article February 2005

ST. LOUIS, Feb. 14 /PRNewswire-FirstCall/ -- Sigma-Aldrich Corporation, a $1.2 billion Life Science and High Technology company, announces commercialization of the TargeTron Gene Knockout System. Developed by Dr. Alan Lambowitz, University of Texas at Austin, and exclusively licensed from Ingex L.L.C., the TargeTron system promises to revolutionize functional genomics and systems biology.

The TargeTron system provides the most robust and simple method available for site-specific disruption of DNA sequences within a host cell genome. In fact, permanent gene disruption is performed in a non-random or targeted manner that does not require host recombination factors to mobilize. The technology exploits the retrohoming ability of group II introns in order to "target" the exact position of gene disruption. An extensively validated TargeTron algorithm designs specific primer sets for use in PCR mutation of the TargeTron Intron. The mutated intron is essentially "re-programmed" to insert itself into the algorithm predicted site of any user-defined gene. Ligation of the PCR product into a TargeTron vector is followed by transformation of the host cell. Expression results in a ribonucleoprotein complex that targets the intron to the precise insertion site within the host genome resulting in gene knockout.

Thus far, the technology has provided greater than 90% successfully targeted insertion rate in prokaryotes. The current TargeTron system has been used to create permanent knockouts across a broad range of bacterial strains such as Escherichia coli, Staphylococcus aureus, Lactococcus lactis, Clostridium perfringens, Shigella flexneri and Salmonella typhimurium. Future systems will be developed for both plant and mammalian targeted gene disruption.

"Group II intron-mediated gene disruption offers advantages over the laborious traditional gene knockout techniques such as randomly integrated transposons and cumbersome recombination methods," commented Keith Jolliff, Global Marketing Manager of Molecular Biology at Sigma-Aldrich. "The cutting- edge TargeTron technology has potential to be the most widely applicable functional genomics tool for creating knockouts across a spectrum of host organisms."

This press release is provided in good-faith by Chiltern Magazine Services Ltd ("CMS") as a service to users of www.telecomsinfo.com. Whilst all care is taken in sourcing and preparing material includede here, any error or incorrect content cannot form the basis for any legal action against the site-owner. Users are advised to check the accuracy of any statements before relying upon them. Likewise, any forward-looking statements made by the author of the press release should be treated as such.

CMS, P&A House, Alma Road, Chesham, Bucks. HP5 3HB, UK
Tel: +44 (0)1494 771734
Fax: +44 (0)1494 778994
e-mail: Editor, CMSinfo

For more information about us, visit CMSinfo.